TABLE 2.
Summary of immunofluorescence staining of whole bacterial cells
Alab49 construct | Growth phase | E64 cysteine protease inhibitor | Antiserum | Immunofluorescence staining scorea |
---|---|---|---|---|
WT | Logarithmic | Absent | Preimmune | 0 |
Logarithmic | Absent | Anti-rFbaA | 3+ | |
Stationary | Absent | Anti-rFbaA | 0 | |
Stationary | Present | Anti-rFbaA | 3+ | |
ΔfbaA | Stationary | Present | Anti-rFbaA | 0 |
ΔfbaAΔpam | Stationary | Present | Anti-rFbaA | 0 |
Δmga | Stationary | Present | Anti-rFbaA | 0 |
ΔspeB | Stationary | Absent | Anti-rFbaA | 3+ |
0, no staining; 3+, strong staining.