TABLE 3.
Effects of genetic deletions on RNA transcript levels of fbaA and flanking genes
| Alab49 construct | Growth phasea | RNA transcript level relative to that in Alab49WTb |
|||
|---|---|---|---|---|---|
| pam | scpA | fbaA | lmb | ||
| ΔfbaA | Logarithmic | ND | 1.59 ± 0.076 | <0.001 | 1.12 ± 0.84 |
| ΔfbaAΔpam | Logarithmic | ND | 1.22 ± 1.06 | <0.001 | 0.78 ± 0.43 |
| Δmga | Logarithmic | 0.012 ± 0.011 | 0.045 ± 0.049 | 0.043 ± 0.027 | ND |
| Stationary | 0.096 ± 0.134 | ND | 0.164 ± 0.171 | ND | |
a
OD600 values for logarithmic cultures ranged from 0.448 to 0.482.
b
Based on two or more independent RNA preparations analyzed by qRT-PCR. ND, not determined.