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The S. aureus purA and purB mutants require exogenous purines for growth. (a to c) Strains were grown in BHI (a), bovine serum (b), or human serum (c). Data are from three independent repeats; error bars represent standard errors. Circles, JE2; squares, JE2-purB; triangles, JE2-purA. (d) Growth of strains on CDM agar plates with or without adenine (20 μg ml−1) and guanine (20 μg ml−1) after 24 h of aerobic incubation at 37°C.
