TABLE 1.
Tn library mutants identified as having an altered phenotype on human blood agar
| Categorya | Protein ID | NARSA ID | Protein name | Growth phenotype in: |
Hemolysis phenotype in: |
||
|---|---|---|---|---|---|---|---|
| Human blood | Rabbit blood | 5% human blood + Columbia agar | 5% sheep blood + Columbia agar | ||||
| A1 | SAUSA300_1889 | NE522 | Adenylosuccinate lyase (PurB) | Reduced growth | Reduced growth | Increased hemolysis | Reduced hemolysis |
| SAUSA300_0017 | NE529 | Adenylosuccinate synthetase (PurA) | Reduced growth | Reduced growth | Increased hemolysis | Slightly increased hemolysis | |
| SAUSA300_0698 | NE821 | para-Aminobenzoate synthase, glutamine amidotransferase, component II (PabA) | Highly reduced growth | Slightly reduced growth | —b | — | |
| A2 | SAUSA300_0955 | NE460 | Autolysin (Atl) | Opaque colony | Opaque colony | Increased hemolysis | — |
| SAUSA300_0193 | NE1253 | N-Acetylmuramic acid-6-phosphate etherase (MurQ) | — | — | Increased hemolysis | Increased hemolysis | |
| SAUSA300_2326 | NE1304 | Transcription regulatory protein (AraC) | — | — | Reduced hemolysis | — | |
| SAUSA300_0899 | NE1315 | Adaptor protein (MecA) | — | — | Reduced hemolysis | Slightly reduced hemolysis | |
| SAUSA300_1305 | NE1391 | Dihydrolipoamide succinyltransferase (OdhB) | Slightly reduced growth | Slightly reduced growth | Increased hemolysis | Increased hemolysis | |
| SAUSA300_0320 | NE1775 | Triacylglycerol lipase (LipA) | Slightly reduced growth | — | Increased hemolysis | — | |
| B | SAUSA300_1989 | NE95 | Accessory gene regulator protein B (AgrB) | — | — | — | Reduced hemolysis |
| SAUSA300_1991 | NE873 | Accessory gene regulator protein C (AgrC) | — | — | Slightly reduced hemolysis | No hemolysis | |
| SAUSA300_0690 | NE1296 | Sensor histidine kinase (SaeS) | — | — | — | No hemolysis | |
| SAUSA300_1058 | NE1354 | Alpha-hemolysin (Hla) | — | — | — | No hemolysis | |
| SAUSA300_1992 | NE1532 | Accessory gene regulator protein A (AgrA) | — | — | Reduced hemolysis | No hemolysis | |
| SAUSA300_0691 | NE1622 | DNA-binding response regulator (SaeR) | — | — | — | No hemolysis | |
a
A1, strains with a defect in growth on human blood agar, which were investigated further; A2, strains with altered hemolysis on human blood agar, which were investigated further; B, strains expected to show a hemolysis phenotype, which were not explored further.
b
—, no difference from the control strain, JE2.