TABLE 4.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype/markers | Source or reference |
|---|---|---|
| Strains | ||
| S. aureus strains | ||
| SH1000 | Functional rsbU+ derivative of 8325-4 | 51 |
| RN4220 | Restriction-negative, modification-positive strain | 52 |
| USA300 JE2 | USA300 LAC strain cured of plasmids p01 and p03 | 11 |
| SJF4669 | SH-pabA::spc pdp::ery | This study |
| SJF4670 | SH-pabA::spc nupC::ery | This study |
| SJF4671 | SH-thyA::ery | 27 |
| SJF4678 | SH-pabA::spc tdk::ery | This study |
| JC006 | JE2-pabA, pJC002 inserted at lipase; pabA+ Eryr Linr Tetr | This study |
| JC007 | JE2-pabA, pKASBAR inserted at lipase; Eryr Linr Tetr | This study |
| JC010 | SH-pabA, pJC002 inserted at lipase; pabA+ Eryr Linr Tetr | This study |
| JC011 | SH-pabA, pKASBAR inserted at lipase; Eryr Linr Tetr | This study |
| LS1 | Spontaneous murine arthritis isolate | 53 |
| LS1 ΔisdE ΔhtsA | LS1 derivative; ΔisdE ΔhtsA | 33 |
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80 lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pKASBAR | Hybrid vector of pCL84 and pUC18 for integration into S. aureus lipase gene (geh), attP; Tetr (S. aureus); Specr (E. coli) | 46 |
| pJC002 | pKASBAR containing the pab operon, with pabA, pabB, and pabC, and upstream control elements; Tetr | This study |