TABLE 1.
Day after NTHi instillation | Mean score for NTHi colonization per ME |
|
---|---|---|
CCL3−/− mice | WT mice | |
0 | 0 | 0 |
1 | 3.2 | 3.3 |
2 | 1.4 | 1.7 |
3 | 3 | 2.3 |
5 | 1.5 | 0.8 |
10 | 1.2 | 0.1 |
14 | 1.4 | 0.6 |
21 | 0.3 | 0 |
Bacterial colonization of MEs was evaluated by using a semiquantitative analysis of the presence of bacteria on culture plates. A 1-μl loop from the ME was sequentially streaked onto the four quadrants of each culture plate, where 0 indicates no CFU, 1 indicates one quadrant with CFU, 2 indicates two quadrants with CFU, 3 indicates three quadrants with CFU, and 4 indicates CFU in all four quadrants. Data represent means of results from at least 6 MEs per time point and strain.