FIG 3.

The full-length M1 and truncated M3 rocA alleles, but not the truncated M18 rocA allele, complement a rocA mutant when expressed from a multicopy plasmid. (A) Schematic of the RocA protein and of the locations of the truncations in the M3 (blue asterisk) and M18 (green asterisk) rocA alleles. RocA has six putative transmembrane domains (TM), a putative dimerization and histidine phosphotransfer domain (DHp), and a putative histidine kinase-like catalytic domain (HATPase). (B) TaqMan-based quantitative RT-PCR analysis to assay the abilities of the M1, M3, and M18 rocA alleles to complement the naturally rocA mutant M3 isolate MGAS10870. Shown are the averages (±standard deviations) of duplicate samples run in triplicate. The asterisks highlight statistical significance relative to the parental M3 isolate containing empty vector (t test; *, P < 0.001).