TABLE 1.
Overview of the GAS strains used in this study
| GAS strain | Description | Reference |
|---|---|---|
| MGAS2221 | Representative M1 clinical isolate (covRS+ rocA+) | 30 |
| 2221ΔrocA | MGAS2221 derivative in which rocA has been replaced with a spectinomycin resistance cassette | 24 |
| 2221ΔrocA::rocAM1 | Complemented derivative of 2221ΔrocA in which the spectinomycin resistance cassette has been replaced with a functional rocA allele from serotype M1 GAS | 24 |
| MGAS2221(pDCBB) | MGAS2221 derivative containing the empty chloramphenicol-resistant vector pDCBB, a phoZ-less derivative of pDC123 | 32 |
| MGAS2221(pRocA-M1) | MGAS2221 derivative containing the plasmid pRocA-M1, which is a pDCBB-based plasmid containing a functional rocA allele from M1 GAS | This study |
| 2221ΔrocA(pDCBB) | 2221ΔrocA derivative containing the empty chloramphenicol-resistant vector pDCBB | This study |
| 2221ΔrocA(pRocA-M1) | 2221ΔrocA derivative containing the plasmid pRocA-M1, which is a pDCBB-based plasmid containing a functional rocA allele from M1 GAS | This study |
| 2221ΔcovS | MGAS2221 derivative that contains a mutant covS allele due to the deletion of a single nucleotide | 30 |
| MGAS10870 | Representative M3 clinical isolate (covRS+ rocA mutant) | 46 |
| 10870(pDCBB) | MGAS10870 derivative containing the empty chloramphenicol-resistant vector pDCBB | 57 |
| 10870(pRocA-M1) | MGAS10870 derivative containing the plasmid pRocA-M1, which is a pDCBB-based plasmid containing a functional rocA allele from M1 GAS | This study |
| 10870(pRocA-M3) | MGAS10870 derivative containing the plasmid pRocA-M3, which is a pDCBB-based plasmid containing a nonfunctional rocA allele from M3 GAS | This study |
| 10870(pRocA-M18) | MGAS10870 derivative containing the plasmid pRocA-M18, which is a pDCBB-based plasmid containing a nonfunctional rocA allele from M18 GAS | This study |
| 10870(pRocA-M11–409) | MGAS10870 derivative containing the plasmid pRocA-M11–409, which is a pDCBB-based plasmid containing a truncated rocA allele from M1 GAS | This study |
| 10870(pRocA-M11–328) | MGAS10870 derivative containing the plasmid pRocA-M11–328, which is a pDCBB-based plasmid containing a truncated rocA allele from M1 GAS | This study |
| 10870(pRocA-M11–276) | MGAS10870 derivative containing the plasmid pRocA-M11–276, which is a pDCBB-based plasmid containing a truncated rocA allele from M1 GAS | This study |
| 10870(pRocA-M11–221) | MGAS10870 derivative containing the plasmid pRocA-M11–221, which is a pDCBB-based plasmid containing a truncated rocA allele from M1 GAS | This study |
| 10870(pRocA-M11–89) | MGAS10870 derivative containing the plasmid pRocA-M11–89, which is a pDCBB-based plasmid containing a truncated rocA allele from M1 GAS | This study |