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. 2017 Aug 6;63(5):519–525. doi: 10.1262/jrd.2017-035

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This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 1. — Comparison of the effects of various concentrations of estradiol (E2), estrone (E1), or estriol (E3) in media containing 1 nM gonadotropin-releasing hormone (GnRH) on luteinizing hormone (LH) secretion from cultured bovine anterior pituitary (AP) cells. The concentrations of LH in the control cells (cultured in medium alone; shown as white bars) were averaged and set at 100%, and the mean LH concentration for each treatment group is expressed as a percentage of the control value. + P < 0.05 and ++ P < 0.01 compared to the control; * P < 0.05 and ** P < 0.01 compared to GnRH alone.