Figure 1.
IL-17A producing cells in the lung following Cm infection. BALB/c mice were intranasally infected with Cm (1 × 103 IFUs). Lung mononuclear cells were isolated and intracellular IL-17A, as well as surface markers, were analyzed at various time points by flow cytometry. (a) Percentage of IL-17A-producing-CD3+γδ+ T, CD3+CD4+ T, CD3+CD8+ T cells and CD3− NK cells in gated lymphocytes. (b) Percentage of IL-17A-producing cells in gated γδ+CD3+T cells, CD4+CD3+ T cells, CD8+CD3+ T cells and DX5+CD3− cells. (c) Percentage of IL17A producing CD3+γδ+ T, CD3+CD4+ T, CD3+CD8+ T cells and CD3− NK cells in total IL-17A-producing cells. (d) Absolute number of IL-17A-producing γδT, CD4+ T, CD8+ T and NK cells. Three independent experiments with four mice in each group were tested, and a representative experiment is shown.