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. 2016 Oct 31;14(10):850–861. doi: 10.1038/cmi.2016.53

Figure 4.

Figure 4

Depletion of γδT cells significantly affected Th17 but not Th1 responses during Cm infection. Mice were depleted of γδT cells and intranasally infected with Cm, as described in the legend to Figure 3. (a) Percentage of IL-17A-produced CD4+ T cells in the lung tissues were determined by intracellular cytokine staining (left panel) and the absolute number of IL-17A-produced CD4+ T cells in the lung tissues was calculated using total lung mononuclear cell number and the ratio of the IL-17A+CD4 cells determined by flow cytometry (right panel). (b) IFNγ production was measured in the culture supernatants of the lung mononuclear cells (left panel) and lung tissue homogenates (right panel) by ELISA at day 7 and day 14 p.i. (c) Percentage of IFNγ-producing CD4+ T cells (left panel) and CD8+ T cells (right panel) in the lung tissue was determined by intracellular cytokine staining. Three independent experiments with four mice in each group were tested, and a representative experiment is shown. The results are shown as the mean±s.d. *P<0.05.