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. 2017 Nov 1;27(13):913–930. doi: 10.1089/ars.2016.6844

FIG. 9.

FIG. 9.

Exo-miR-15a from pancreatic β-cells to the retina during T2D. We have generated pancreatic β-cell-specific miR-15a−/− mice. (A) On the left, a representative blot of the Tg(insulin II promoter, Ins2) Cre. On the right, a representative blot of the microRNA cluster 30 (Mirc30) at 14qC3 of the mouse genome. (B) qPCR data for miR-15a expression in retina from Ins2-cre transgene mice (WT) or pancreatic β-cell-specific miR-15a−/− mice (β cell-specific 15a KO). Both groups were 18-week HFD-fed animals. The data were normalized with β-actin expression. qPCR data examining the (C) miR-15a expression and (D) miR-451a expression in the exosomal fraction of blood from Ins2-cre transgene mice (WT) or pancreatic β-cell-specific miR-15a−/− mice (β cell-specific 15a KO). The data were normalized with equal RNA concentration (5 ng). *p > 0.05 versus WT.