Figure 2.

Antitumor function of the NCR2-based chimeras. (A) Human primary lymphocytes were transduced with a retroviral vector encoding either h4428z, s4428z, 44tm28z, and truncated CD34 (control). Transduced T cells were cocultured with different tumor lines or normal PBLs as indicated. IFNγ secreted in the coculture supernatant was measured by ELISA. These results are presented as mean + SEM (n = 5, with three different donors). (B) s4428z- or CD34-transduced T-cells were cocultured with the indicated target cells and IL2 (right panel) and TNFα (left panel) concentration secreted in the culture supernatant was determined by ELISA. These results are presented as mean + SEM (n = 5, with three different donors). (C) Transduced PBLs with either s4428z or CD34 cells were cocultured with tumor lines as indicated and analyzed by flow cytometry for 4-1BB expression gated on the CD8⁺ population. The percentage of positive cells is shown and the dotted line represents the marker staining of the control. These results are representative of three independent experiments with different donors and the difference between s4428z and CD34 was found to be statistically significant (p < 0.05, calculated using a Student’s paired t-test).