Figure 3.

Function of the s4428z chimera against cells derived from primary melanoma culture. (A) Human primary lymphocytes were transduced with a retroviral vector encoding s4428z and truncated CD34 (control). Transduced T cells were cocultured with cells derived from primary melanoma cultures (M#3 and M#14) as well as two control tumor lines (DU145 positive and Colo205 negative controls). IFNγ, IL2, and TNFα secreted in the coculture supernatant was measured by ELISA. These results are presented as mean + SEM (n = 3, with two different donors). (B) Transduced PBLs with either s4428z or CD34 cells were cocultured with the indicated targets and analyzed by flow cytometry for 4-1BB expression gated on the CD8⁺ population. The percentage of positive cells is shown and the dotted line represents the marker staining of the control. These results are representative of three independent experiments with different donors and the difference between s4428z and CD34 was found to be statistically significant (p < 0.05, calculated using a Student’s paired t-test).