Figure 4.

Cell size in T. pseudonana under experimental evolution conditions. (a) cell diameter measured as average particle diameter by coulter counter of cells grown for 300 generations at 22 °C (green) and 9 °C (blue). Error bars represent s.d. of measurements taken from biological triplicate samples. (b) Transcript abundance of silacidins; Protein IDs 7349, 23671, 6681, 12137 and 23686 found to be differentially requlated in TSD lines under late exponential and silicon-starved conditions and with expression patterns apparently similar to silacidins; cell wall genes silaffin 1 and silaffin 3; and an actin-like gene used as a housekeeping gene in transcriptomes of the same cell lines harvested after 300 generations at 22 °C (T300 22 °C, green), 9 °C (T300 9 °C, blue) and from batch culture nutrient conditions prior to the onset of the evolution experiment (T0 22 °C, green, with black outline). Error bars represent s.d. between transcriptomes sequenced from biological replicate samples (T300 22 °C n=5, T30 9 °C n=4).