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. 2017 Jul 11;11(11):2492–2509. doi: 10.1038/ismej.2017.103

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Copyright © 2017 The Author(s)

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Relative fitness of ΔpvdD and ΔpvdDΔpchEF mutants in pure culture (grey bars) and in mixed culture with an isogenic wild type (white bars) in ex vivo pig lung+ASM after 96 h of growth. Bars show means of eight replicates spread across two replica experiments, with associated 95% confidence interval. (ANOVA eliminating experimental block: genotype F_1,27=2.24, P=0.146; presence/absence of the wild type: F_1,27=10.7, P=0.003; interaction term: F_1,27=0.137, P=0.714). Neither strain had a relative fitness signficantly different from 1 in pure culture (post hoc t-tests, P=0.074 for ΔpvdD, P=0.472 for ΔpvdDΔpchEF). In co-culture, relative fitness was not significantly different from 1 for ΔpvdD (P=0.074), but <1 for ΔpvdDΔpchEF (P=0.007). In both panels, results of post hoc tests for relative fitness=1 are shown with *=P=0.007; n.s.=not significant.