Figure 1.

Flow diagram summarizing how the data required for high-quality descriptions of uncultivated taxa can be obtained. Representative bioinformatics software or technology to use for each task is shown on the connecting lines. Dashed lines denote data or tasks that could be omitted for minimum quality descriptions (see text for details). HISH-SIMS, halogen in situ hybridization and secondary ion mass spectroscopy; MAR-FISH, microautoradiography and fluorescence in situ hybridization; MLSA, multilocus sequence analysis.