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Trefoil factors and cytokines transcriptional regulation upon H. pylori infection in AGS-AC1 clone. Real-time PCR analysis of IL-8 (A), IL-6 (B), TFF1 (C), TFF2 (D) and TFF3 (E) mRNA after 36 h of H. pylori infection in AGS-AC1 cells induced or not with Doxycycline to express TFF1. Experiments were carried out at least in triplicate and data are expressed as mean ± SD (t-test, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001, ****p ≤ 0.0001).
