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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Thromb Haemost. 2010 Sep 30;105(1):21–30. doi: 10.1160/TH10-07-0446

Figure 2. The CUB-1 consensus cysteine mutants impaired ADAMTS-13 secretion.

Figure 2

ADAMTS-13 cDNAs were transiently transfected into CHO cells and their expression evaluated in the conditioned media, cell lysates, and ECM 72 hrs after transfection by immunoblotting. Secretion of four consensus cysteine mutants was significantly reduced in the conditioned media (top panel), but not to ECM (bottom panel). In contrast, C1275S was secreted in the amount comparable to WT. Intracellular pool of the ADAMTS-13 mutants was comparable to that of WT (middle panel). Actin was used as sample loading controls. The figure was a representative of five separate experiments.