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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Thromb Haemost. 2010 Sep 30;105(1):21–30. doi: 10.1160/TH10-07-0446

Figure 7. Binding of rADAMTS-13 to immobilized VWF.

Figure 7

VWF multimers purified from human cryoprecipitate were immobilized onto the wells of microtiter plate overnight at 4°C. rADAMTS-13 was then incubated with immobilized VWF for 30 min and bound ADAMTS-13 detected by a polyclonal antibody against the fourth TSP-1 motif (clone 156) (ANOVA test, n = 3, p > 0.05 among WT and mutants).