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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: J Immunol. 2017 Mar 15;198(8):3296–3306. doi: 10.4049/jimmunol.1602059

FIGURE 3.

FIGURE 3

Biochemical effects of substrate-selective p38 inhibitors. (A and B) Heat maps from RNASeq showing IPA pathways inhibited by SB203580 alone or SB203580 and UM101 or (A) and those only inhibited by UM101 (B). (C) HeLa cells were pretreated with 50 μM UM101 or 10 μM SB203580 (SB) for 30 min, then treated with anisomycin for 10–60 min, and immunoblotted for phospho-MK2, phospho-STAT-1, and total p38. The means of the band densities from two experiments expressed as percent of total density for all bands are shown below each corresponding band. (D and E) In vitro kinase reaction containing activated p38α, ATP [γ-32P], 5 μM SB203580, or 50 μM UM101, and the indicated substrate were incubated at 37°C for indicated time, separated by SDS-PAGE, and analyzed by phosphorimaging. A representative of three gels (D) and means ± SE of the 120-min band densities expressed as fold-change versus the 5-min control reaction band are shown. *p < 0.0001, †p < 0.01 versus control.