Figure 2.

FnIII-1c and FnEDA synergistically regulate NF-κB translocation to the nucleus. Monolayers of human dermal fibroblasts were serum starved overnight before treatment with FnIII domains, either individually (2 μM) or in combination, for the indicated amount of time. Nuclear fractions were collected, and NF-κB was visualized by Western blot (A). Blots were quantified by using densitometry and values for NF-κB were normalized to Lamin A/C, which served as a loading control. Resulting values were normalized to the control (FnIII-10n) at each time point. The theoretical expected additive effect of combining FnEDA and FnIII-1c is represented by the open dashed line bar. Data are presented as fold change at 60 min (B). Statistical analysis was performed by using a Student's t-test. *p < 0.05. Data are presented as the mean ± S.E.M. (n = 3). NF-κB, nuclear factor kappa-light chain enhancer of activated B cells.