Figure 5.

Fibronectin modules and LPS do not synergistically regulate IL-8 expression in human monocytes. THP-1 monocytes were rinsed with serum-starving medium before treatment with 2 μM of the designated FnIII domains and 2 μg/mL LPS, alone or in combination. Conditioned medium was collected after 4 h; then, it was analyzed by ELISA for IL-8. The theoretical expected additive effect of combining FnEDA and FnIII-1c is represented by the open dashed line bar. Data represent the mean ± S.E.M. (n = 3, A/B; n = 5, C). Statistical analysis was performed by using a Student's t-test. NS, not significant.