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. 2017 Sep;12(9):1507–1518. doi: 10.4103/1673-5374.215263

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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qPCR analysis of 10 genes shown to be differentially expressed by Solexa sequencing.

PCR was performed on 10 genes randomly selected from differentially expressed genes identified by Solexa sequencing. Data are expressed as the mean ± SD (n = 4; one-way analysis of variance followed by Dunnett's t-test). Each data point represents the average from three tests, and error bars represent the standard deviations. Ftl1: Ferritin light chain 1; Cdk5r2: cyclin-dependent kinase 5 activator 2; Omg: oligodendrocyte-myelin glycoprotein; Mif: macrophage migration inhibitory factor; Uqcrh: ubiquinol-cytochrome c reductase hinge protein; Ncdn: neurochondrin; Syp: synaptophysin; Gstp1: glutathione S-transferase P; Tgfb3: transforming growth factor beta-3; Thbs1: thrombospondin-1. The experiment was repeated three times. qPCR: Quantitative polymerase chain reaction. I: Ftl1; II: Cdk5r2; III: Omg; IV: Mif; V: Uqcrh; VI: Ncdn; VII: Syp; VIII: Gstp1; IX: Tgfb3; X: Thbs1.