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. 2017 Sep;12(9):1519–1528. doi: 10.4103/1673-5374.215264

Figure 5.

Figure 5

Effect of PED and JSK on NGF protein and mRNA expression determined by immunohistochemistry and qRT-PCR.

(A) Representative images of immunohistochemistry of NGF in the rat spinal cord at 3, 7, and 14 days post-injury. Scale bar: 250 μm. (B) Quantification of NGF immunoreactivity. (C) NGF mRNA analyzed by two-step fluorescent quantitative RT-PCR increased in the SCI + PED and SCI + JSK (M) groups compared with the SCI group. Data are expressed as the mean ± SD (n = 3, one-way analysis of variance and the least significant difference test). ##P < 0.01, vs. SCI group; ††P < 0.01, vs. SCI + PED group. PED: Prednisolone 60 mg/kg/d; JSK: Jisuikang, JSK (H): 50 g/kg/d, JSK (M): 25 g/kg/d, and JSK (L): 12.5 g/kg/d. SCI: Spinal cord injury; qRT-PCR: quantitative real-time polymerase chain reaction.