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. 2017 Sep;12(9):1519–1528. doi: 10.4103/1673-5374.215264

Figure 6.

Figure 6

Effect of PED and JSK on BDNF protein and mRNA expression by immunohistochemistry, western blot assay, and qRT-PCR.

(A) Representative images of immunohistochemistry of BDNF in the rat spinal cord at 3, 7, and 14 days post-injury. Scale bar: 250 μm. (B) Quantification of BDNF immunoreactivity. (C) Western blot assay of BDNF protein expression. (D) Quantification of BDNF protein densitometry. (E) NGF mRNA expression was quantified by two-step fluorescent quantitative PCR. Data are expressed as the mean ± SD (n = 3, one-way analysis of variance and least significant difference test). #P < 0.05, vs. SCI group; ††P < 0.01, vs. SCI + PED group. PED: Prednisolone 60 mg/kg/d; JSK: Jisuikang, JSK (H): 50 g/kg/d, JSK (M): 25 g/kg/d, and JSK (L): 12.5 g/kg/d. SCI: Spinal cord injury; qRT-PCR: quantitative real-time polymerase chain reaction.