Figure 1.

Overview of mesoscale modeling approach used to determine boundary conditions for microscale models. Mesoscale models were built from 30-μm thick serial histological image stacks of sheep ONH with 4.4 μm per pixel in-plane resolution. Polarized light microscopy was used to determine the collagen fiber orientation and the local collagen density within each section.³⁴ Colors in the polarized light microscopy images correspond to the fiber angle. These were mapped onto the finite element mesh and used to determine the stiffness and direction of anisotropy for each element. IOP-induced hoop stress corresponding to 30 mm Hg was applied to the boundary of the model to obtain displacement boundary conditions for the microscale models. The displacements corresponding to the actual location of each microscale model within its corresponding mesoscale model were used. The red box shown on the bottom-left image corresponding to the location of the model shown in Figure 2 is shown as an example.