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. 2017 Oct 12;23:4885–4894. doi: 10.12659/MSM.906997

Figure 2.

Figure 2

MiR-150 negative regulated the expression of RAB11A in thyroid cells. (A) Targeting sites of miR-150 shown in human RAB11A mRNA 3′ UTRs used TargetScan Human 7.1 and the mutational 3′ UTRs of RAB11A. (B, C) EGFP intensity of K1 and TPC-1 cells with indicating treatment was determined by spectrophotometer, and the value of control group (pcDNA3 or ASO-NC) was set to one. (D) RAB11A mRNA levels in K1 and TPC-1 cells with indicating treatment were measured by RT-qPCR assays. (E) RAB11A protein levels in K1 and TPC-1 cells transfected with pri-miR-150 or ASO-miR-150 and respective controls were determined by western blot assays; ns, not significant; * p<0.05; ** p<0.01; *** p<0.001.