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MiR-150 negatively regulated the WNT/β-catenin pathway. (A, B) K1 cells were transfected with the indicated combinations of pcDNA3 and pcDNA3, pcDNA3 and pri-miR-150, pri-miR-150, and pRAB11A. Western blot assay (A) was used to detect the expression level of β-catenin, p-GSK3β, C-myc, and cyclin D. Immunofluorescence assay (B) was used to examine the nuclear distribution of β-catenin; * p<0.05; ** p<0.01; *** p<0.001.
