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. 2017 Aug 18;14(4):4983–4988. doi: 10.3892/ol.2017.6769

Figure 3.

Figure 3.

NEAT1 promotes E2F3 expression in part through competitive binding miRNA-377-3p. The relative level of miR-377-3p in A549 and H1299 (A). The luciferase report assay demonstrated that miR-377-3p could reduce the intensity of fluorescence in A549 (B) and H1299 (C) transfected with the NEAT1-WT vector. Luciferase reporter assays indicated that luciferase activity generated by the reporter vector with E2F3-3′-WT decreased more than 50% after co-transfected with miR-377-3p in both A549 (D) and H1299 (E). Overexpression of miR-377-3p inhibited NEAT1 expression (F). Overexpression of miR-377-3p inhibited E2F expression in A549 (G). Inhibiting NEAT1 resulted in a decrease of E2F3 in A549 (H). P<0.05.