Figure 2.

S100A9 induces tongue SCC cell invasion through its interaction with CD147. Cell invasiveness was evaluated in vitro using a Matrigel invasion assay. (A) SAS and (B) HSC-3 tongue SCC cells were plated in the inserts in serum-free medium with or without S100A9 (100 nM), Ab (10 µg/ml), IgG (10 µg/ml) or a combination of S100A9 with Ab or S100A9 with IgG. Untreated cells were used as a control. The results are shown as fold-changes in invasion relative to the control. Both (A) SAS and (B) HSC-3 cell invasiveness increased in response to S100A9 stimulation, and the addition of Ab reversed this increase. The experiment was repeated three times, and fold invasiveness relative to control was expressed as the mean ± standard deviation. *P<0.05 compared with the NoTX group. S100A9, S100 calcium-binding protein A9; SCC, squamous cell carcinoma; CD147, cluster of differentiation 147; Ab, antibody; IgG, immunoglobulin G; NoTX, no treatment.