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. 2017 Aug 14;130(16):1809–1818. doi: 10.1182/blood-2017-03-772962

Figure 1.

Figure 1.

Cebpb−/− mice lack Ly6C−monocytes. (A) Cebpb mRNA expression in purified hematopoietic populations (n = 3). (B) Expression of CD115 and CD135 in BM cells among c-kit+ lineage marker (CD11b, Gr-1, CD11c, Ter119, IL-7R, CD3, CD19, and NK1.1)− cells. The frequencies of MDPs (CD115+ CD135+) and cMoPs (CD115+ CD135−) among lineage− BM cells are shown in the right panel (n = 5). Data were pooled from 4 independent experiments. (C,E) Gating strategy to define Ly6C+ and Ly6C− monocytes in the BM (C) and PB (E). Lineage marker (Ly6G, B220, CD4, CD8, and NK1.1) − CD11b+ CD115+ monocytes were subdivided on the basis of Ly6C expression. (D,F) Frequencies of Ly6C+ and Ly6C− monocytes in the BM (D) and PB (F) of WT or Cebpb−/− mice (n = 10 for BM and n = 19 for PB). Data were pooled from 7 independent experiments and are presented as the mean ± standard deviation (SD). **P < .01, ***P < .001. Numbers indicate the percentage of cells in the adjacent boxed area. FSC, forward scatter; Lin, lineage.