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(A) Effect of PA and OA on MDA-MB-231 cell apoptosis. (B) Effect of free fatty acids on autophagy. The MDA-MB-231 cells were cultured with 0.5% BSA or 250 µM BSA-bound PA or OA for 24 h. The levels of LC3 and autophagy flux were determined by western blot analysis. A representative blot is shown. *P<0.05 and ***P<0.001 vs. negative control. BSA, bovine serum albumin; PA, palmitate acid; OA, oleic acid; BFA, Bafliomycin A1; LC3, light chain 3.
