Figure 3.

miR-182 downregulates FOXF2 by directly targeting its 3′UTR and regulates EMT. (A) The putative binding site of miR-182 in the 3′UTR of FOXF2. Mutant was generated in the 3′UTR of FOXF2, named as MT. (B) Analysis of luciferase activity. MCF-7 and MDA-MB-231 cells were cotranfected with miR-182 or miR-control, and FOXF2 3′UTR WT or MT. Firefly luciferase activity was normalized to Renilla luciferase activity. The data are from at least three independent experiments. *P<0.05. (C) Western blot analysis of EMT and FOXF2 protein levels in MCF-7 and MDA-MB-231 cell lines. (D) RNA specific to FOXF2 effects on MDA-MB-231 cell invasion and migration. miR, microRNA; FOX, forkhead-box (F2); EMT, epithelial-mesenchymal transition; MT, mutant-type; WT, wild-type; 3′UTR, 3′-untranslated region.