Figure 4.

The interaction between RACK1 and WEE1 in HGC27 cells (A). The protein expression of WEE1 in HGC27 cells. The protein expression of WEE1 was detected in HGC27 cells at 48 h after transfection of pcDNA3.1-RACK1 and pcDNA3.1 empty vector (B). The interaction between RACK1 and WEE1 in HGC27 cells was confirmed by co-immunoprecipitation. HGC27 cells were collected and lysed, protein was extracted, prewashed and incubated with RACK1 antibody, added the protein A+G beads. After denaturation, the PVDF membrane was detected with WEE1 antibody for Western blotting (C). The location of RACK1 and WEE1 in HGC27 cells was observed by indirect immunofluorescence assay. Scale bar, 200 µm.