Table I.
Sequences of primers.
| Primer | Experiment | Type | Sequence (5′-3′) | Product size | Annealing temperature |
|---|---|---|---|---|---|
| NCCRP1 | qRT-PCR | Forward | AAAGCTCCAGCAGAACCAAA | 104 bp | 60°C |
| Reverse | TAATGGCTGGTTGTTCGTCA | ||||
| Bisulfite | Forward | TTTAGTTAATTTTAGTTTTGTGAAAT | 282 bp | 64°C | |
| sequencing | Reverse | CCACTCCTCCAACAACAACTAC | |||
| GAPDH | qRT-PCR | Forward | GAAGGTGAAGGTCGGAGTC | 226 bp | 60°C |
| Probe | CAAGCTTCCCGTTCTCAGCC | ||||
| Reverse | GAAGATGGTGATGGGATTTC |
NCCRP1, non-specific cytotoxic cell receptor protein 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.