Figure 2.

DNMT1 is a target of miRNA-148a. (A) TargetScan was used to predict miRNA-148a target genes. The DNMT1 gene 3′UTR included 7 sequential pairing bases with the 5′ of miRNR-148a, indicating that DNMT1 may be a potential target of miRNA-148a. (B) In order to confirm that DNMT1 is a target, an miRNA-148a mimic or inhibitor was transfected into MCF7 cells. At 48 h after transfection, the detection of DNMT protein levels by western blot analysis indicated that the expression of DNMT1 decreased upon miRNA-148a mimic transfection and increased upon miRNA-148a inhibitor transfection. DNMT1, DNA methyltransferase 1; miRNA, micro RNA; 3′UTR, 3′ untranslated region.