Table 1.

Efficiency of DPC formation by 5fC within NCPs.

5fC position and base pair	histone H4 mutants in NCP	DPC% at equilibriuma
5fC89-G	WTb	20.1 ± 0.7
	Del 1-20c	4.2 ± 0.2
	K5, 8, 12, 16, 20Rd	14.7 ± 4.1
	CapNe	6.7 ± 0.2
	CapN-K5, 8, 12, 16, 20Rf	3.3 ± 0.2
5fC89-A	WT	16.6 ± 4.6
5fC89-T	WT	21.4 ± 0.7
5fC74-G	WT	3.2 ± 0.1
5fC3-G	WT	3.6 ± 0.1
5fC45-G	WT	14.8 ± 0.3

^aYields are averages ± standard deviations of at least three experiments.

^bWild type histone H4.

^cHistone H4 without the N-terminal 1-20 amino acids.

^dH4 with Lys 5, 8, 12, 16, 20 to Argmutations.

^eH4 with capped N terminal by thiazolidine (see Scheme 1).

^fH4 with both capped N terminal and Lys 5, 8, 12, 16, 20 to Arg mutations.