Figure 3.

Effect of knocking down PTEN on cell apoptosis and maspin levels in 22Rv1 cells. (A) Paclitaxel induced apoptosis in 22Rv1 cells when PTEN was knocked down. (B) Paclitaxel induced change in maspin protein and mRNA levels in 22Rv1 cells when PTEN was knocked down. 22Rv1 cells were transfected with PTEN siRNA or control siRNA. At 48 h subsequent to transfection, cells were treated with 10 nM paclitaxel for 48 h. The cells were harvested and subjected to (A) apoptosis and (B) immunoblotting or RT-PCR analysis. (C and D) Densitometric analysis of maspin protein and mRNA level shown in (B), respectively. Results from three independent experiments were quantified. Error bars indicate the standard deviation among three individual experiments. *P<0.01. PTEN, phosphatase and tensin homolog; RT-PCR, reverse transcription-polymerase chain reaction; siRNA, small interfering RNA.