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. 2017 Aug 23;14(4):4758–4766. doi: 10.3892/ol.2017.6791

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Transfection of CEACAM1-4S in breast cancer cells suppressed invasion and migration. (A) Western blot analysis verified the transfection efficiency of CEACAM1-4S in BT549 and Hs578T cells. Transwell invasion assays were performed following CEACAM1-4S overexpression in (B) BT549 and (C) Hs578T cells. Representative images are shown (magnification, ×100), and data are presented as the mean ± standard deviation of three independent experiments. ***P<0.001. Effects of CEACAM1-4S on cell migration were assessed by wound healing assays in (D) BT549 and (E) Hs578T cells following CEACAM1-4S transfection (magnification, ×200). Relative closure of the cell-free areas is presented as the mean ± standard deviation from three separate experiments, **P<0.01; *P<0.05. CEACAM1-4S, carcinoembryonic antigen-related cell adhesion molecule 1-4S.