Figure 5.

CEACAM1-4S induces a gene expression pattern consistent with epithelial-mesenchymal transition reversal. (A) Western blot analysis of E-cadherin, N-cadherin and vimentin in CEACAM1-4S-transfected BT549 and Hs578T cells. CEACAM1-4S overexpression led to the induction of E-cadherin and abrogation of N-cadherin and vimentin. (B) Western blot analysis of p-STAT3, p-Smad2, total STAT3 and Smad2/3 in CEACAM1-4S-transfected BT549 and Hs578T cells. Exogenous expression of CEACAM1-4S decreased the phosphorylation of STAT3 and Smad2 but had no marked effect on the levels of total STAT3 and Smad2/3. Immunofluorescence images of controls and breast cancer cells overexpressing CEACAM1-4S. (C) BT549 and (D) Hs578T cells were stained for E-cadherin and vimentin (magnification, ×200). CEACAM1-4S-transfected cells exhibited weaker staining for vimentin and stronger staining for E-cadherin compared with corresponding controls. CEACAM1-4S, carcinoembryonic antigen-related cell adhesion molecule 1-4S; p-, phosphorylated; STAT3, signal transducer and activator of transcription.