Figure 7.

Vasoactivity of infused packed red cell supernatant/plasma.

(A) Experimental time line for packed red cell supernatant infusions. Rats were stabilised for 30 minutes (min) after surgery, and blood gasses were drawn as indicated (BG 1 and BG 2). Supernatant (1.6 mL) of packed red blood cells stored for either 4 or 39 days was infused for 40 min, after which the rats were followed for 1 hour (n=5). (B) Change in mean arterial pressure (MAP) over time after packed red blood cell (RBC) supernatant infusion and 60-min follow up. (C) Average percentage peak increase in MAP after infusion of packed RBC supernatants (RBC sup) (p=0.003). (D) Correlation (solid line) between packed red blood cell supernatant haeme concentration and percentage increase in MAP after 40-min infusion of packed red blood cell supernatant stored for either four days (black solid circle) or 39 days (black solid square; r²=0.65). Each data point was obtained from a separate rat infusion experiment, in a different rat (2 groups of n=5). All values are displayed as mean standard error of mean (SEM). Student t-test was used to compare the 2 groups of rats. Avg.: average; conc.: concentration. (Adapted with permission from Donadee et al., 2011⁸⁵).