Figure 5. Inhibition of RNA and protein synthesis in AML cell lines treated with AZD1208.

OCI-AML-3 (circles), KG-1a (squares), MOLM-16 (diamonds), MOLM-13 (open triangles) MV-4-11 (inverted open triangle) cell lines were incubated with 0.1% DMSO or 3 μM AZD1208 for 24 h, then 1 h prior to harvesting the cells (A) [³H]uridine was added to the cell culture or (B) [³H]leucine was added to the cell culture as described in Materials and Methods. The results represent an average of triplicate experiments ± SEM. mTOR pathway proteins. (C) mTOR signaling as a driver of protein synthesis and cell size. (D) General scheme of mTORC1 and mTORC2 complex components. Protein phosphorylation decreased by Pim kinase inhibition with AZD1208 are shown in bold.