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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Cell Metab. 2017 Oct 3;26(4):633–647.e7. doi: 10.1016/j.cmet.2017.09.009

Figure 3. Breast CSCs exhibit an enhanced mitochondrial respiratory phenotype.

Figure 3

(A) OCRs were determined in cells sorted by ALDH activity (upper panel) or grown as mammospheres or in adherent condition (lower panel). (B) MDA-MB-436 and SUM159PT cells sorted or grown as in A were stained with Mitotracekr Red CMXRos and then analyzed by flow cytometry (*p<0.005). (C) ROS levels were determined by ROS-Glo as described in Experimental Procedures (*p<0.05, **p<0.005). (D) Cells were stained with MitoSOX Red and analyzed by flow cytometry (*p<0.005, **p<0.0005). (E) Cells were sorted by flow cytometry upon intensity of Mitotracker Red CMXRos (upper) and then seeded in mammosphere assays for 7 days (* p<0.05). Data are represented as mean ± SD.