Figure 5.

Coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) of lung sections. Lavaged lungs from control and GM-CSF KO animals were subjected to CARS and SHG microscopy. A and B show CARS images that confirm the significant increases in lipid droplets present in GM-CSF lungs. C and D show the respective overlays of images A with E, and B with F and define the spatial relationships between lipid accretion and fibrotic changes. E and F show the SHG images of the same areas, which show a pattern of increased collagen fibers in the GM-CSF KO lungs.