Table 1.
CARS Quantification
| CARS Image Intensity ± SD (per Image FOV) | Size (% of Total Number of Lipid Droplets) |
||||
|---|---|---|---|---|---|
| < 1 µm2 (Small) | 1–5 µm2 (Medium) | > 5 µm2 (Large) | |||
| Lavaged lung | Control | 107.7 ± 34.4 | 72.3 | 25.2 | 2.5 |
| GM-CSF KO | 300.7 ± 34.2 | 70.7 | 25.2 | 4.1 | |
| Liver | Control | 193.1 ± 49.9 | 80.3 | 18.9 | 0.8 |
| GM-CSF KO | 413.5 ± 235.3 | 68.7 | 26.6 | 4.7 | |
From three samples each of control and granulocyte–macrophage colony-stimulating factor knockout (GM-CSF KO) lung and liver tissues, 10 fields of view (FOV) were selected (i.e., 30 for each group) and subjected to coherent anti-Stokes Raman scattering (CARS) imaging. Upon subsequent image analyses, the distribution of lipid droplets was characterized using arbitrary size ranges broadly defined as small, medium, and large. The CARS image intensity reflects the lipid concentration, permitting semiquantitative assessment of the total lipid change. Increases in total lipid and droplet sizes are readily apparent in both the lungs and livers of GM-CSF KO animals.