Figure 3.

Representative Western blots and quantitative densitometry analyses for eIF2a and ATF4 proteins. DM mice (CHOP^−/−/GADD34^−/−) and WT mice were exposed to either IH or RA for 6 weeks. (A) eIF2α phosphorylation was increased by IH in WT-IH mice but not in DM-IH mice. Total proteins were isolated from individual vWATs from individual mice and immunoblotted against p-eIF2α and total eIF2α. Representative immunoblots and a summary of signal intensity ratios across experimental groups are shown. (B) Representative immunoblot for ATF4 protein and β-actin as the loading control in vWAT, and summary of signal intensities across experimental groups after normalization to β-actin as the loading control. Significant increases in ATF4 expression occurred in WT-IH but not in DM-IH. Results are expressed as fold change and represent mean ± SD, n = 8/experimental group. Data are presented as mean ± SD. ATF4, activating transcription factor 4; eIF2, eukaryotic translation initiation factor 2.