Table 1.
Summary of GLP1-ELP fusions and their characterization
| Construct Name | Xaa Composition (A:V:L) |
VPGXG Repeats (N) |
Tt at Injected Conc. (°C) |
Molecular Weight (kDa) |
Rh (nm) |
In Vitro EC50 (nM) |
In Vitro EC50 95% CI (nM) |
|---|---|---|---|---|---|---|---|
| Transition Temperature Series | |||||||
|
| |||||||
| GLP1-ELP15.5°C | 0:6:4 | 120 | 15.5 | 53,446 | 5.3 ± 0.1 | 26.0 | 14.4 to 47.0 |
| GLP1-ELP21.4°C | 0:1:0 | 120 | 21.4 | 52,772 | 6.2 ± 0.1 | 13.0 | 7.5 to 22.4 |
| GLP1-ELP30.2°C | 5:5:0 | 120 | 30.2 | 51,209 | 6.0 ± 0.1 | 7.1 | 2.9 to 17.3 |
| GLP1-ELP35.5°C | 6:5:0 | 120 | 35.5 | 50,873 | 6.3 ± 0.1 | 3.8 | 2.2 to 6.4 |
| GLP1-ELP48.8°C | 9:1:0 | 120 | 48.8 | 49,742 | 5.9 ± 0.1 | 6.6 | 4.4 to 9.9 |
|
| |||||||
| Molecular Weight Series | |||||||
|
| |||||||
| GLP1-ELP20.0kDa | 0:1:0 | 40 | 31.5 | 20,013 | 4.4 ± 0.1 | 12.5 | 6.4 to 24.3 |
| GLP1-ELP35.8kDa | 3:7:0 | 80 | 29.9 | 35,840 | 5.3 ± 0.1 | 5.7 | 1.8 to 17.7 |
| GLP1-ELP67.5kDa | 4:6:0 | 160 | 30.6 | 67,476 | 7.4 ± 0.1 | 11.8 | 5.1 to 27.2 |
| GLP1-ELP98.7kDa | 5:5:0 | 240 | 32.3 | 98,664 | 8.0 ± 0.3 | 10.1 | 6.2 to 16.4 |
|
| |||||||
| μSPECT-CT Samples | |||||||
|
| |||||||
| GLP1-ELPopt | 4:6:0 | 160 | 30.6 | 67,476 | 7.4 ± 0.1 | 11.8 | 5.1 to 27.2 |
| GLP1-ELPsol | 1:0:0 | 160 | 54.6 | 64,783 | 7.4 ± 0.1 | 11.0 | 5.0 to 24.0 |