Table 1. Diet compositions, calculated and analysed nutrient specifications (g/kg) in experimental diets.
| Ingredients/Diet | Spl1 | spl | sPl | spl | spL | spl | Pe | PE |
|---|---|---|---|---|---|---|---|---|
| Maize | 738 | 630 | 522 | 570 | 619 | 679 | 375 | 668 |
| Soybean meal | 207 | 238 | 270 | 250 | 230 | 218 | 503 | 36 |
| Soya protein isolate | 0.0 | 27.5 | 55.0 | 27.5 | 0.0 | 0.0 | 10.0 | 100 |
| Casein | 0.0 | 39.8 | 79.7 | 39.8 | 0.0 | 0.0 | 25.0 | 134 |
| Soybean oil | 0.30 | 0.15 | 0.00 | 20.2 | 40.4 | 20.4 | 0.00 | 0.00 |
| Lysine HCl | 1.20 | 0.73 | 0.25 | 0.53 | 0.80 | 1.00 | 0.50 | 0.00 |
| Methionine | 1.20 | 3.03 | 4.85 | 3.08 | 1.30 | 1.25 | 4.80 | 4.90 |
| Threonine | 0.20 | 2.45 | 4.70 | 2.40 | 0.10 | 0.15 | 1.20 | 8.20 |
| Valine | 0.00 | 0.15 | 0.30 | 0.15 | 0.00 | 0.00 | 0.60 | 0.00 |
| Arginine | 0.00 | 1.25 | 2.50 | 1.25 | 0.00 | 0.00 | 0.00 | 5.00 |
| Salt | 1.50 | 1.60 | 1.70 | 1.75 | 1.80 | 1.65 | 1.80 | 1.60 |
| Sodium bicarbonate | 2.60 | 1.83 | 1.05 | 1.68 | 2.30 | 2.45 | 2.10 | 0.00 |
| Limestone | 9.90 | 10.35 | 10.80 | 10.25 | 9.70 | 9.80 | 9.90 | 11.7 |
| Dicalcium phosphate | 11.50 | 9.75 | 8.00 | 9.85 | 11.70 | 11.60 | 8.00 | 8.00 |
| Phytase2 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 |
| Choline chloride 60% | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Vitamin-mineral premix3 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Cellulose | 3.50 | 10.6 | 17.6 | 39.2 | 60.7 | 32.1 | 35.2 | 0.00 |
| CeliteTM | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 |
| Calculated | ||||||||
| Metabolisable energy (MJ/kg) | 12.75 | 12.67 | 12.58 | 12.67 | 12.75 | 12.75 | 11.04 | 14.12 |
| Protein | 160 | 230 | 300 | 230 | 160 | 160 | 300 | 300 |
| Lipid | 32 | 28 | 25 | 46 | 67 | 49 | 21 | 28 |
| Starch | 500 | 428 | 355 | 388 | 420 | 460 | 260 | 450 |
| Calcium | 8.7 | 8.7 | 8.7 | 8.7 | 8.7 | 8.7 | 8.7 | 8.7 |
| Total phosphorus | 5.3 | 5.3 | 5.3 | 5.2 | 5.2 | 5.3 | 5.9 | 4.6 |
| Phytate P | 2.4 | 2.4 | 2.4 | 2.3 | 2.2 | 2.3 | 3.0 | 1.8 |
| Non-phytate P | 3.0 | 3.0 | 2.9 | 3.0 | 3.0 | 3.0 | 2.9 | 2.9 |
| Lysine4 | 8.5 | 13.0 | 17.5 | 13.0 | 8.5 | 8.5 | 17.5 | 17.5 |
| Methionine | 3.7 | 6.6 | 9.6 | 6.6 | 3.7 | 3.7 | 9.0 | 10.1 |
| Methionine + cysteine | 6.3 | 9.7 | 13.0 | 9.7 | 6.3 | 6.3 | 13.0 | 13.0 |
| Threonine | 5.6 | 10.2 | 14.8 | 10.2 | 5.6 | 5.6 | 11.6 | 17.9 |
| Tryptophan | 1.6 | 2.5 | 3.4 | 2.6 | 1.7 | 1.7 | 3.6 | 3.2 |
| Arginine | 9.4 | 14.0 | 18.6 | 14.2 | 9.8 | 9.6 | 19.0 | 18.2 |
| Sodium | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 | 1.8 |
| Potassium | 7.7 | 8.1 | 8.5 | 8.1 | 7.7 | 7.7 | 12.5 | 4.4 |
| Chloride | 2.0 | 1.9 | 1.9 | 1.9 | 2.0 | 2.0 | 2.0 | 1.7 |
| Analysed 5 | ||||||||
| Starch | 423 | 334 | 323 | 313 | 359 | 358 | 243 | 349 |
| Protein | 173 | 254 | 278 | 261 | 165 | 183 | 297 | 285 |
| Lipid | 25 | 26 | 23 | 39 | 59 | 46 | 22 | 29 |
| Dry matter | 858 | 859 | 864 | 865 | 861 | 861 | 859 | 863 |
1Experimental diet: S = starch, P = protein, L = lipid, E = energy. Capital, lowercase letters and letters with underline meant high, low and medium dietary concentration, respectively. For example, diet Spl contained high starch concentration and low protein and lipid concentrations. Diet PE contained high protein and high energy, respectively.
2Axtra PHY TPT was supplemented at 1000 FTU/kg
3The vitamin-mineral premix supplied per tonne of feed: [MIU] retinol 12, cholecalciferol 5, [g] tocopherol 50, menadione 3, thiamine 3, riboflavin 9, pyridoxine 5, cobalamin 0.025, niacin 50, pantothenate 18, folate 2, biotin 0.2, copper 20, iron 40, manganese 110, cobalt 0.25, iodine 1, molybdenum 2, zinc 90, selenium 0.3
4Digestible basis
5As-is basis and protein was analysed as N x 6.25; lipid was analysed by Soxhelt extraction