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. 2017 Oct 20;12(10):e0186542. doi: 10.1371/journal.pone.0186542

Fig 1. S. senegalensis sperm motility after cryopreservation.

Fig 1

Three different internal cryoprotectants (CPAs) were tested: dimethyl sulfoxide (DMSO), dimethyl formamide (DMF) and methanol (MetOH), at 10% concentration, supplemented with two different external CPAs: 1% bovine albumin serum (BSA) and 10% egg yolk (EY). The following CASA parameters were recorded: (A) percentage of motile cells and (B) velocity according to the actual path (VCL; μm/s). Motility parameters were considered at 15, 30, 45 and 60 seconds post-activation. All results were expressed as a mean of percentages ± SE of 6–10 pools of 10 individual males for each treatment. A general linear model with the Bonferroni correction was used (p< 0.05). Different letters show significant differences between conditions (p<0.05).